TM Pst1 Protéine

N° du produit ABIN2469152

Cette protéine Recombinant est produite dans Yeast (Saccharomyces Cerevisiae).

Aperçu rapide pour TM Pst1 Protéine (ABIN2469152)

Antigène: TM Pst1

Type de proteíne: Recombinant

Origine: Saccharomyces cerevisiae

Source: Yeast (Saccharomyces Cerevisiae)

Application: Screening Assay (ScA), ELISA, Western Blotting (WB)

Pureté: ~95 %

Détail du produit

Information d'application

Indications d'application: This recombinant protein can be used for WB, ELISA, and other assays to screen and identify mannose-specific antibodies.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Lot specific, TBS or PBS

Conseil sur la manipulation: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Stock: -80 °C

Stockage commentaire: Store in working aliquots at -70 °C.

Détail du antigène

Antigène: TM Pst1

Sujet: Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction (1). Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor (2). Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at around 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (?och1 ?mnn1 ?mnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified glycan-specific PGT antibodies (3). Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows high affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses (4).

Poids moléculaire: 100 kDa on SDS-PAGE

ID gène: 851625

OMIM: 17380259

UniProt: Q12355